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Saprophyticus usually gives white colonies in blood agar, but colonies can be yellow to orange Entire, very glossy, smooth, opaque, butyrous, and convex. Based on the gram stain result, why can we rule this organism down as the cause in the patient Colonies capable of utilizing citrate as a carbon source produce a local increase in ph, changing the color of the medium from green to blue
Only citrate positive organisms will grow on this medium. Positive results for acetoin production, benzidine test, catalase, urease, acid production from Fructose, glucose, glycerol, maltose, sucrose, trehalose and turanose. When exposed to hydrogen peroxide, the bacteria produce bubbles of oxygen gas, confirming their catalase activity.
The major test reaction to use in staphylococcus identification is the coagulase test reaction, which divides the genus staphylococcus into 2 groups—coagulase negative species and coagulase positive species.
The urinary test strips may yield a falsely negative result in the case of s Saprophyticus infection (due to the absence of nitrite formation), and treatment with fosfomycin is often ineffective. Obtain cultures (with susceptibilities) as appropriate for the site of infection Blood cultures may be positive for staphylococcal species, even when results from other cultures are negative.
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